Gene disruption by homologous recombination in the Xylella fastidiosa citrus variegated chlorosis strain

• Author(s): Gaurivaud, Patrice; Mariano, A.; Monteiro, Patricia; Palma, Rene; Souza, Leonardo; Virgilio, A.;
• Abstract: Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model. Integration of replicative plasmids by homologous recombination between the cloned truncated copy of bga and the endogenous gene was produced by one or two crossover events leading to beta-galactosidase mutants. A promoterless chloramphenicol acetyltransferase gene was used to monitor the expression of the target gene and to select a cvaB mutant.
• Publication Date: Feb 2002
• Journal: Applied And Environmental Microbiology