Antioxidant activity of polyphenols from seeds of Vitis amurensis in vitro

• Author(s): Alves, Simone; Chen, Y.; Hano, Yoshio; Tan, R.; Taro, N.;
• Abstract: AIM: To study the antioxidant action of five polyphenols (+) catechin, procyanidin B-2, procyanidin B-2, procyanidin Bg 3-O-gallate, and amurensisin isolated from the seeds of Vitis amurensis. METHODS: The mouse liver homogenate lipid peroxidation assay was applied for the evaluation of the antioxidant activity in vitro. RESULTS: (+)Catechin, procyanidin B-2, procyanidin B-5, procyanidin B-5 3-O-gallate, and amurensisin showed antioxidant activity with the IC50 values of 0.47, 0.25, 0.10, 0.02, and 0.03 mmol/L, respectively. The IC50 value of vitamin E used as a positive control was 0.13 mmol/L. The structural activity relationship was also analyzed. Procyanidins carrying a galloyl group possessed higher anti-lipid peroxidation activities. All dimers were found to be more potent than the non-galloylated momomer such as (+)catechin. However, the activity of the 4--6 linked dimer seemed more preferable than 4--8 linked dimer. CONCLUSION: Procyanidin B-5, procyanidin B-5 3-O-gallate, and amurensisin showed a more antioxidant activity than vitamin E did, and their activity is dependent on their substitution and polymerization patterns.
• Publication Date: Jul 2000
• Journal: Acta Pharmacologica Sinica