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Pierce's Disease
Research Updates

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What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

Multilocus simple sequence repeat markers for differentiating strains and evaluating genetic diversity of Xylella fastidiosa

  • Author(s): Barros, Samuel; Civerolo, Ed; Francis, Marta; Hu, R.; Lin, Hong; Walker, Andrew;
  • Abstract: A genome-wide search was performed to identify simple sequence repeat (SSR) loci among the available sequence databases from four strains of Xylella fastidiosa (strains causing Pierces disease, citrus variegated chlorosis, almond leaf scorch, and oleander leaf scorch). Thirty-four SSR loci were selected for SSR primer design and were validated in PCR experiments. These multilocus SSR primers, distributed across the X. fastidiosa genome, clearly differentiated and clustered X. fastidiosa strains collected from grape, almond, citrus, and oleander. They are well suited for differentiating strains and studying X. fastidiosa epidemiology and population genetics.
  • Publication Date: Aug 2005
  • Journal: Applied And Environmental Microbiology

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