Characterization of cell lines developed from the glassy-winged sharpshooter, Homalodisca coagulata (Hemiptera : Cicadellidae)

• Author(s): Do, Z.; Hagler, Jacquelyn; Hammock, Bruce; Kamita, Shizuo; Samra, A.;
• Abstract: Four continuous cell lines were established from the embryos Of the glassy-winged sharpshooter, Homalodisca coagulata (Say), an economically important insect vector of bacterial pathogens of grape, almond, citrus, oleander, and other agricultural land ornamental plantings. The cell lilies were designated GVISS-Z10, GWSS-Z15, CWSS-G3, and GWSS-LH. The GWSS-Z10, GWSS-Z15, aid GWSS-G3 lines were cultured in Ex-Cell 401 medium supplemented with 10% fetal bovine serum (FBS), whereas the GWSS-LH line was cultured in LH medium supplemented with 20% FBS. The cell lilies were characterized in terms of their morphology, growth, protein composition, and polymerase chain reaction-amplification patterns of their chromosomal deoxyribonucleic acid. The population doubling times of GWSS-Z10, GWSS-Z15, GWSS-G3, and GWSS-LH were 46.2, 90.9, 100.3, and 60.2 h, respectively. These lines should be useful for the study of insect-pathogenic viruses of leafhoppers, aphids, treehoppers, and other related insects as well as plant-pathogenic viruses that are transmitted by these insects.
• Publication Date: May 2005
• Journal: In Vitro Cellular & Developmental Biology-Animal