COMPARATIVE PROTEOMIC ANALYSIS OF STEM TISSUE AND XYLEM SAP FROM PIERCES DISEASE RESISTANT AND SUSCEPTIBLE GRAPEVINES

• Author(s): Fritschi, Felix; Lin, Hong; Walker, Andrew; Yang, Litao;
• Abstract: Analyses of host plant resistance / susceptibility mechanisms to Xylella fastidiosa (Xf) infection are critical for understanding host-pathogen interactions. Proteomic analyses of stem tissue and xylem sap samples were initiated to complement genomic approaches employed in elucidating Pierces disease resistance mechanisms. Samples from one highly resistant and two susceptible grape genotypes were collected at multiple time points post-inoculation from control and Xf-inoculated plants. Two-dimensional gel electrophoresis revealed numerous proteins that were differentially expressed and dependent on plant genotype and/or inoculation treatment. Proteins identified by oMALDI-TOF comprised a wide range of functional types. The importance of these proteins with respect to host-pathogen interactions will be investigated further.
• Publication Date: Nov 2006
• Journal: 2006 Pierce's Disease Research Symposium