MULTI-LOCUS SIMPLE SEQUENCE REPEAT (SSR) MARKERS FOR GENOTYPING AND ASSESSING GENETIC DIVERSITY OF XYLELLA FASTIDIOSA IN CALIFORNIA

• Author(s): Lin, Hong; Civerolo, Ed; Groves, Russell; Walker, Andrew;
• Abstract: We have designed and developed Xylella fastidiosa (Xf) Simple Sequence Repeat (SSR) primers. Thirty-four of them have been validated and are available to public (Lin et al. 2005). These primers are Xf-specific and powerful for detecting polymorphism among and within crop-associated Xf strains and can be used for Xf genotyping, population structure and genetic diversity studies. Recently, we used fluorescent-labeled primers for PCR and an ABI 3100 genetic analyzer in combination with our rapid sample preparation protocol to create a high-throughput Xf pathogen diagnostic and genetic analysis platform. We used this marker system to study the geographic population structures of grape Xf strains in California. We also used this marker system as a tool to study interactions between Vitis and Xf in Pierces disease (PD) resistant and susceptible grapes.
• Publication Date: Dec 2005
• Journal: 2005 Pierce's Disease Research Symposium