SOLUBLE FORMS OF AN ANTI-XYLELLA ANTIBODY AND STRAINS OF ALCALIGENES XYLOSOXIDANS DENITRIFICANS CAPABLE OF SECRETING THEM

• Author(s): Lampe, David; Lauzon, Carol; Miller, Thomas; Richards, Frank; Rose, Bob;
• Abstract: Several methods were used to create soluble forms of a single chain antibody (scFv S1) that binds to the surface of the grape strains of Xylella fastidiosa (Xf). S1 fused to a pelB leader and secreted from E. coli. These forms were not secreted correctly and could not bind Xf in an ELISA. Maltose binding protein fusions of S1 were soluble and could be used to detect Xf in an ELISA. We also successfully secreted S1 from Alcaligenes xylosoxidans denitrificans (Axd) using a leader sequence that directed S1 to the periplasmic space. Strains of Axd that secrete anti-Xylella factors are being developed for use in a strategy to prevent the spread of Xf.
• Publication Date: Dec 2005
• Journal: 2005 Pierce's Disease Research Symposium