EVALUATION OF RESISTANCE POTENTIAL IN THE GLASSY-WINGED SHARPSHOOTER USING TOXICOLOGICAL, BIOCHEMICAL, AND GENOMICS APPROACHES

• Author(s): Byrne, Frank; Federici, Brian; LeVesque, Cynthia; Toscano, Nick;
• Abstract: We used microarray expression profiling to identify genes that were differentially regulated in glassy-winged sharpshooters in response to treatment with the pyrethroid insecticide esfenvalerate. Targets were prepared from insects treated at both (LD)50 and sub-lethal doses. Of the 1,536 cDNAs in the array, only eight were differentially regulated in response to esfenvalerate. Of these, three aligned significantly with ferritin, lysozyme i-1, and polynucleotide phosphorylase. Additional (pyrethroids have been included in our bioassay program. In topical application bioassays, LD)50(s for bifenthrin and fenpropathrin were 1.2 and 3.6 ng/insect, respectively. Esfenvalerate, with an LD)50 of 0.75 ng/insect, is the most toxic of the (pyrethroids we have tested against the GWSS. Populations of insects have been selected with these insecticides at LD)50 and sublethal doses for microarray analyses.
• Publication Date: Dec 2005
• Journal: 2005 Pierce's Disease Research Symposium