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Pierce's Disease
Research Updates

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What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

DEVELOPMENT OF SSR MARKERS FOR GENOTYPING AND ASSESSING THE GENETIC DIVERSITY OF XYLELLA FASTIDIOSA IN CALIFORNIA

  • Author(s): Lin, Hong; Walker, Andrew; Civerolo, Ed;
  • Abstract: Recently available genomic sequences of four Xylella fastidiosa strains (PD, CVCD, ALSD and OLSD) facilitate genome wide searches for identifying Simple Sequence Repeat (SSR) loci. Sixty SSR loci were selected for SSR marker development. We designed and validated 34 SSR primers with good reliability and specificity. These SSR primers showed various levels of polymorphism with average 11.3 alleles per locus among 43 Xylella fastidiosa isolates. These multi-locus SSR markers, distributed across the entire genome, are a useful tool for pathogen genotyping, population genetics and molecular epidemiology studies.
  • Publication Date: Dec 2004
  • Journal: 2004 Pierce's Disease Research Symposium

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