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Pierce's Disease
Research Updates

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What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

ROLE OF TYPE I SECRETION IN PIERCES DISEASE

  • Author(s): Gabriel, Dean;
  • Abstract: Xylella fastidiosa Temecula sequence information reveals no type III, but two type I secretion systems, both dependent on a single tolC homologue. Marker exchange mutagenesis using pGEM-T as delivery vector and nptII as marker was employed to generate tolC disruptions. PCR and Southern blot analyses confirmed marker exchange at the tolC locus. Grape (var. Carignane) plants inoculated with mutant (tolC::nptII) strains exhibited no symptoms of PD, indicating that pathogenic ability of PD strains may be dependant on tolC and type I secretion. Complementation assays using tolC in the mutant strains are in progress to help confirm this hypothesis.
  • Publication Date: Dec 2004
  • Journal: 2004 Pierce's Disease Research Symposium

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