Pierce's Disease
Research Updates

piercesdisease.cdfa.ca.gov

What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples


  • Author(s): Brady, JA; Castaneda-Gill, JM; Faske, JB; King, JL; Mitchell, FL;
  • Abstract: We report an inexpensive, high-throughput method for isolating DNA from insect and plant samples for the purpose of detecting Xylella fastidiosa infection. Existing methods often copurify inhibitors of DNA polymerases, limiting their usefulness for PCR-based detection assays. When compared to commercially available kits, the method provides enhanced pathogen detection at a fraction of the cost. (C) 2011 Elsevier B.V. All rights reserved.
  • Publication Date: Sep 2011
  • Journal: Journal Of Microbiological Methods