MAP BASED IDENTIFICATION AND POSITIONAL CLONING OF XYLELLA FASTIDIOSA RESISTANCE GENES FROM KNOWN SOURCES OF PIERCES DISEASE RESISTANCE IN GRAPE

• Author(s): Riaz, Summaira; Walker, Andrew;
• Abstract: Genetic mapping efforts continue in the 9621 population (D8909-15 x F8909-17). Xylella fastidiosa (Xf) resistance is segregating in this population both the Vitis arizonica and a V. arizonica/V. champinii are resistant. We have increased the mapping population size from 116 to 188 individuals, thus increasing recombination frequencies and taking advantage of 96-well plate based techniques. We are adding the original 375 AFLP markers to the additional 72 individuals. Three hundred and ten SSR markers have been tested of which 192 amplified and 165 were polymorphic in the population these have been added to the map. Fourteen of 20 EST markers from Doug Adams lab were also added. We have also added 16 EST derived SSR markers from Doug Cooks database of ESTs with the goal of adding 100 to 150 more by June. Xf resistance maps to linkage group 14. There are two flanking SSR markers on one side and an SSR and AFLP marker on the other side of the Xf resistance locus derived from F8909-17.
• Publication Date: Aug 2003
• Journal: 2003 Pierce's Disease Research Symposium