MARKER-ASSISTED SELECTION FOR RESISTANCE TO XYLELLA FASTIDIOSA: ACCELERATED BREEDING OF PIERCES DISEASE RESISTANT GRAPES

• Author(s): Krivanek, Alan; Riaz, Summaira; Walker, Andrew;
• Abstract: Efforts at identifying genetic markers tightly linked to Xylella fastidiosa (Xf) resistance are continuing. These efforts are primarily focused on the 9621 mapping population (D8909-15 x F8909-17) and are in cooperation with fine-scale mapping efforts discussed in our report Map based identification and positional cloning of Xylella fastidiosa resistance genes from known sources of Pierces disease resistance in grape. This project is adding 200 SSR markers to the 9621 map and has positioned Xf resistance from the male parent F8909-17 on the lower arm of linkage group 14, where Xf resistance is flanked by multiple markers. Resistance from the female parent D8909-15 maps as a quantitative trait locus. The addition of additional SSR marker is expected to reduce the distance between SSR markers and Xf resistance to about 2 cM and lead to the development and utilization of very specific sequence characterized amplified region (SCAR) primers. These markers will be applied to populations in the breeding program derived from D8909-15 and F8909-17 and tested for effectiveness in Xf resistance backgrounds derived from other species.
• Publication Date: Aug 2003
• Journal: 2003 Pierce's Disease Research Symposium