Fractal analysis of Xylella fastidiosa biofilm formation

• Author(s): Cotta, MA; Lorite, GS; Moreau, ALD; Rodrigues, CM; Souza, AA;
• Abstract: We have investigated the growth process of Xylella fastidiosa biofilms inoculated on a glass. The size and the distance between biofilms were analyzed by optical images; a fractal analysis was carried out using scaling concepts and atomic force microscopy images. We observed that different biofilms show similar fractal characteristics, although morphological variations can be identified for different biofilm stages. Two types of structural patterns are suggested from the observed fractal dimensions D-f. In the initial and final stages of biofilm formation, D-f is 2.73 + 0.06 and 2.68 + 0.06, respectively, while in the maturation stage, D-f = 2.57 + 0.08. These values suggest that the biofilm growth can be understood as an Eden model in the former case, while diffusion-limited aggregation (DLA) seems to dominate the maturation stage. Changes in the correlation length parallel to the surface were also observed; these results were correlated with the biofilm matrix formation, which can hinder nutrient diffusion and thus create conditions to drive DLA growth. (C) 2009 American Institute of Physics. [DOI: 10.1063/1.3173172]
• Publication Date: Feb 2010
• Journal: Journal Of Applied Physics