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Pierce's Disease
Research Updates

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What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

Genetic transformation of 'Valencia' sweet orange with the cecropin MB39 gene

  • Author(s): Camargo, RLB; de Paoli, LG; Harakava, R; Mendes, BMJ; Mourao, FDA;
  • Abstract: The objective of this work was to produce 'Valencia' sweet orange transgenic plants with the cecropin MB39 gene controlled by a phenylalanine ammonia-lyase gene promoter from citrus in order to direct gene expression in xylem vessels. The genetic transformation was mediated by Agrobacterium tumefaciens with the co-culture of epicotyl segments. Eleven transgenic plants were selected by PCR with the amplification of a 189 bp fragment, which were acclimatized to greenhouse. The integration of the transgene was confirmed in three plants by Southern blot analysis.
  • Publication Date: Nov 2007
  • Journal: Pesquisa Agropecuaria Brasileira

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