DEVELOPING TRANSCRIPTIONAL PROFILES AND MICROARRAY EXPRESSION ANALYSIS OF GRAPE PLANT RESPONSE TO XYLELLA FASTIDIOSA

• Author(s): Lin, Hong; Walker, Andrew;
• Abstract: The goal of the project is to characterize the molecular events in the grape / Xylella fastidiosa (Xf) interaction. We are developing a genomic approach to identify transcriptional pathways correlated with susceptibility and resistance. Highly resistant and susceptible genotypes were selected from a Vitis rupestris x V. arizonica population. Reciprocal suppression subtractive hybridization (SSH) cDNA libraries for both resistant and susceptible genotypes that represent the complete spectrum of gene expression profiles in response to the Xf infection are being constructed. This strategy is efficient for cloning and identifying differentially expressed genes associated with signal recognition/transduction and potentially regulated interactions between pathogens and host plants. Based on annotation results, a subset of candidate genes will be selected for cDNA microarray expression analysis. The information derived from this study will help reveal the details of metabolic pathways of host responses and molecular mechanism(s) of grape resistance and susceptibility to Xf.
• Publication Date: Aug 2003
• Journal: 2003 Pierce's Disease Research Symposium