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Pierce's Disease
Research Updates

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What is Pierce's Disease?

Pierce's Disease is a bacterial infection, which is spread by bugs that feed on grapevines, particularly the "glassy winged sharpshooter." Grapevines that become infected with PD can quickly become sick and die.

glassy-winged sharpshooter

PARATRANSGENESIS TO CONTROL PIERCES DISEASE: ISOLATION AND ANALYSIS OF ANTI-XYLELLA SINGLE CHAIN ANTIBODIES

  • Author(s): Lampe, David; Miller, Thomas; Bextine, Blake; Cooksey, Donald; Lauzon, Carol; Richards, Frank;
  • Abstract: We have used a two methods to attempt to isolate single-chain antibodies (scFv) specific for the surface of the Temecula strain of Xylella fastidiosa. A method using whole X. fastidosa cells to pan a phage display scFv library was successful and we recovered one very specific scFv that strongly interacts with Temecula and less so with the Tulare strain of X. fastidiosa. A method using a purified surface protein (mopB) from Temecula yielded two scFvs that interacted with that protein, but failed to interact with intact cells. Intended uses for these scFvs in a paratransgenesis method to control Pierces disease will be discussed.
  • Publication Date: Aug 2003
  • Journal: 2003 Pierce's Disease Research Symposium

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