Inheritance of RAPD markers in an interspecific F1 hybrid of grape between Vitis quinquangularis and V. vinifera

• Author(s): He, Pu-Chao; Luo, Sulan; Zheng, Xueqin; Zhou, Peng;
• Abstract: Inheritance of randomly amplified polymorphic DNA (RAPD) markers in an interspecific F1 hybrid between Vitis quinquangularis (83-4-96) and V. vinifera (Muscat Rose) were studied. A preliminary screen of the F1 plants and the parents used as the interspecific cross parent was conducted with 280 RAPD primers; of these, 39 primers were polymorphic and reproducible and produced 234 scorable loci. Genomic DNA from 60 individuals of the F1 population was then amplified with these primers; 83.4% of the loci segregated as expected in a Mendelian fashion. According to the segregation patterns, RAPD markers were grouped into three types: (1) normal Mendelian inheritance with segregation ratio of nearly 1:1, 3:1 or 1:0 (non-segregation); (2) deviation from Mendelian segregation ratios; (3) abnormal segregation in low frequency, including non-parental markers which were present in F1 progenies but absent in both parents. RAPD markers presenting in both parents were the major source of non-segregation markers; markers presenting in only one parent mainly showed Mendelian segregation. There were five non-parental markers. Markers showing 1:1 and 3:1 segregation were used for constructing an RAPD molecular linkage map. The results demonstrated that RAPD markers could be used to study the genomes of the wild grapes native to China.
• Publication Date: Jan 2002
• Journal: Scientia Horticulturae (Amsterdam)