Medium-dependent response of grapevine somatic embryogenic cells

• Author(s): Demangeat, Gerard; Joly, D.; Martin, D.; Masson, J.; Perring, M.; This, P.;
• Abstract: A set of novel media, combining significant changes in KNO3, NH4Cl, KH2PO4, CaCl2, MnSO4 and ZnSO4 concentrations have been designed for somatic embryogenesis and plant regeneration in grapevine. Friable and white embryogenic calli, consisting of viable cells, were obtained for Cabernet Sauvignon, Chardonnay, Chasselas, Gamay, Gewurztraminer, Grenache, Merlot, Muscat, Pinot Noir, Portan, Riesling, Syrah and the root-stocks 110 Richter, 3309 Couderc and S04. The sole ammonium content was shown to drive somatic grape cells either in a proliferation phase or in a differentiation pathway, i.e. the transition from a type I callus to type II. For 14 genotypes, numerous torpedo could be initiated which developed within similar to 30 days. The maximal torpedo conversion efficiencies reached 100% in Cabernet Sauvignon and Syrah. Plant regeneration efficiencies ranging from 4.75 +/- 4.92%, for Riesling to 79.25 +/- 20.84%, for Syrah were reproducibly reached within 50-100 days. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.
• Publication Date: Jun 2001
• Journal: Plant Science