ROLE OF TYPE I SECRETION IN PIERCES DISEASE

• Author(s): Gabriel, Dean; Lee, Richard;
• Abstract: In an effort to make knockout mutations of a TolC homologue, marker interruption was attempted using two different vectors, one of them newly constructed for the purpose. Although these vectors worked well in Xanthomonas, repeated efforts to obtain such mutants in X. fastidiosa failed. In an attempt to utilize splice-overlap PCR to obtain marker eviction and generate marker-free exconjugants, a marker-eviction vector was constructed. Again, although the vector and method worked well in Xanthomonas, the method failed in X. fastidiosa. Marker exchange experiments are currently underway.
• Publication Date: Aug 2003
• Journal: 2003 Pierce's Disease Research Symposium