Direct somatic embryogenesis and plant regeneration from leaf, petiole, and stem explants of Golden Pothos

• Author(s): Chen, Jianchi; Henny, R.; Zhang, Q.;
• Abstract: Somatic embryos directly formed at cut edges or on the surface of leaf explants, around cut ends or along side surfaces of petiole and stem explants of Golden Pothos [ Epipremnum aureum ( Linden & Andre) Bunt.] on Murashige and Skoog ( MS) medium supplemented with N-(2-chloro- 4- pyridyl)- N-phenylurea ( CPPU) or N-phenyl-N- 1, 2, 3-thiadiazol-5-ylurea (TDZ) with alpha-naphthalene acetic acid (NAA) and a medium called MK containing MS salts with Kaos vitamins, supplemented with 2.0 mg/l TDZ and 0.2 mg/l NAA. Somatic embryos were also produced on MS medium containing 2.0 mg/l kinetin (KN) and 0.5 mg/l 2,4- dichlorophenoxyacetic acid ( 2,4- D) from leaf and petiole explants, MS medium supplemented with 2.0 mg/ l CPPU and 0.5 mg/ l 2,4- D from petiole and stem explants, and 2.0 mg/ l TDZ and 0.2 mg/ l or 0.5 mg/ l 2,4- D from stem explants. In addition, somatic embryos occurred from stem explants on Chus N-6 medium containing 2.0 mg/ l CPPU and 0.2 mg/ l NAA. Somatic embryos matured and grew into multiple buds, shoots, or even plantlets after 2 - 3 months on the initial culture medium. Germination was optimal on MS medium containing either 2 mg/ l 6-benzylaminopurine ( BA) and 0.2 mg/ l NAA or 2 mg/ l zeatin and 0.2 mg/ l NAA. Shoots elongated better and roots developed well on MS medium with no growth regulators. Approximately 30 - 100 plantlets were regenerated from each explant. The regenerated plants grew vigorously after transplanting to a soil-less container substrate in a shaded greenhouse.
• Publication Date: Feb 2005
• Journal: Plant Cell Reports