Nutritional requirements of Hirsutella rhossiliensis

• Author(s): Chen, S.; Liu, X.;
• Abstract: Six natural media, 20 carbohydrates, 18 nitrogen compounds, and nine vitamins were tested for the growth, sporulation, and spore germination of the nematophagous fungus Hirsutella rhossiliensis in solid and/or liquid cultures. Variations in nutritional requirements existed among the fungal isolates. In general VA, CMA and PDA were good media for growth, and MEA, VA and YDA were good for sporulation of all six isolates tested. Glycogen was the best carbon source for growth and spore germination of the three tested isolates ATCCA6487, OWVT-1 and JA16-1. No isolate could utilize D-(+)-xylose. The best carbon source for sporulation was D-(+)-trehalose for ATCC46487, D-sorbitol for OWVT-1, and D-(+)-cellobiose for JA16-1. Casein was the best nitrogen source for growth of ATCC46487 and OWVT-1, while peptone was the best for JA16-1. Spore germination of all isolates was well supported by most nitrogen compounds but was inhibited by L-cystine. No significant difference in sporulation of ATCC46487 was observed among the nitrogen sources. DL-threonine was the best nitrogen source for sporulation of OWVT-1, and L-phenylalanine was the best for JA16-1. Vitamins generally enhanced the fungal growth and sporulation, with thiamine having the greatest influence. However, excluding some vitamins individually from the medium containing all other test vitamins increased growth and/or sporulation of certain isolates.
• Publication Date: Jun 2001
• Journal: Phytopathology