Minimum inhibitory concentrations of terpene on growth of Xylella fastidiosa strains

• Author(s): Chang, Chung-Jan; Franklin, L.;
• Abstract: MICs of terpene AC-2 on growth of 11 strains of X. fastidiosa representing 5 grape strains, 2 sycamore strains, and 1 strain each of peach, plum, pecan, and oleander were studied. Concentrations of 500, 250, and 125 ppm of terpene AC-2 were prepared in sterile water. X. fastidiosa was grown in PW agar at 30 C. An aliquot of 0.5 mL of each strain was added to 0.5 mL of each terpene solution or sterile water in a sterile test tube. The final concentrations of terpene AC-2 were 250, 125, and 62.5 ppm, respectively. This treated cell suspension was incubated for 24 hrs at 30degreeC before the color-changing units were determined by a 10-fold serial dilution in fresh PW to 10-9. All culture tubes were incubated for 20 days before final readings were taken. The MICs, defined as the lowest concentrations in which no cells survived the treatment, were 125 ppm for 4 grape strains, 2 sycamore strains and 1 peach strain, and 62.5 ppm for strains from grape, plum, pecan, and oleander. Terpene AC-2 may be used as a control agent for diseases induced by X. fastidiosa
• Publication Date: Jun 2002
• Journal: Phytopathology