Development of an enzyme-linked immunosorbent assay for the detection of the fungicide fenarimol

• Author(s): Kim, Y.; Kyung, K.; Lee, Eun; Lee, J.; Park, S.;
• Abstract: To develop an enzyme-linked immunosorbent assay for the fungicide fenarimol, two synthesized haptens, haptens-1 and -2, and the purchased 4,4-DDA were conjugated to carrier proteins (BSA, KLH, and OVA). Polyclonal antibodies raised against hapten-1,2-KLH conjugates in rabbits and the coating antigens of hapten-1,2-BSA conjugates, hapten-2-OVA conjugate, and 4,4-DDA-BSA conjugate were screened and selected for the homologous and/or heterologous ELISA formats. Two competitive indirect ELISAs were selected: assays I and II. The optimized ciELISAs of assays I and I I showed average IC50 values of fenarimol of 5.4 and 9.4 ng/mL, detection ranges of 1.1 -25.9 and 1.1 -82.7 ng/mL, and lowest detection limits of 0.3 and 0.3 ng/mL, respectively. The cross- reactivities with several structurally related compounds indicated the importance of the steric fitness in the antigen -antibody interaction. Recoveries of fenarimol from apple and pear samples spiked with the analyte by assay I were in the range of 93-113% by simple extraction, concentration, and dilution. This assay could be a convenient and supplemental analytical tool for monitoring fenarimol residues in environmental and agricultural samples.
• Publication Date: Dec 2004
• Journal: Journal Of Agricultural And Food Chemistry