Direct seeding of grapevine somatic embryos and regeneration of plants

• Author(s): Aman, M.; Gray, D.; Jayasankar, S.; Li, Zhijian;
• Abstract: Somatic embryos of grapevine (Vitis vinifera L.) Chardonnay were produced from liquid suspension cultures. Mature somatic embryos were blot dried briefly in the laminar flow hood and germinated directly in Magenta GA-7 VesselsTM containing one of the following potting media: (1) sand, (2) commercial potting mixture (CPM), or (3) CPM overlaid with sand. Each vessel containing 20 ml of distilled water and the potting medium was sterilized by autoclaving for 30 min and cooled overnight before inoculating the somatic embryos. Five somatic embryos were placed in each vessel under aseptic conditions. The vessels were closed and incubated at 26+-2degreeC, 16 h photoperiod at 75 mumol s-1 m-2 light intensity. Results revealed that CPM overlaid with sand was best for plant development. There was more contamination of somatic embryos on pure CPM. Since direct seeding bypasses at least two subcultures in agar medium, it has implications for use of somatic embryos as synthetic seeds for clonal plant production. This study shows that somatic embryos of grapevine can be handled with some of the convenience of seeds, emphasizing the feasibility for further automating in vitro plant production, which might be especially useful for new varieties where propagation material is limited.
• Publication Date: Jul 2001
• Journal: In Vitro Cellular And Developmental Biology Plant