Somatic embryogenic line induction and maintenance of grapevines

• Author(s): Lu, Jiang; Xu, Xia;
• Abstract: Somatic embryogenesis is a rapid propagation method and an important tool for genetic transformation and somatic hybridization. An experiment was initiated to induce somatic embryogenic lines from grapevines. Embryogenic lines were obtained from immature ovules extracted from berries of muscadine grape cultivars Summit and Tara as well as bunch grape cultivars Autumn Royal, Crimson Seedless, and Fantasy Seedless when cultured on three different media with or without plant growth regulators (PGR). The induction of embryogenic lines was highly genotype-dependent and thus far was confined to these cultivars. However, the embryogenic lines from Summit and Tara were stable maintained on Murashige & Turcker (MT) medium supplemented with 1 muM 2, 4-D and 1 muM NAA while the embryogenic lines from Autumn Royal, Crimson Seedless, and Fantasy Seedless grew better on CP medium supplemented with 0.2 muM BAP and 1muM NOA. Plant regeneration was successfully achieved from those embryogenic lines when transfer them to the Woody Plant (WP) medium supplemented with 1 muM BA.
• Publication Date: Aug 2003
• Journal: Hortscience