Research Symposium Proceedings

• Monitoring The Seasonal Incidence Of Xylella Fastidiosa In Glassy-Winged Sharpshooter Populations
  Project Leader: Steve Castle USDA, ARS, Western Cotton Research Lab Phoenix, AZ 85040 Cooperators: Nilima Prabhaker and Nick Toscano University of California Riverside, CA 92521

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• Development Of An Artificial Diet For The Glassy-Winged Sharpshooter
  Project Leader: Thomas A. Coudron USDA, ARS, BCIRL Columbia, MO 65203 Cooperators: Joe Patt USDA, ARS, KDLG Subtropical Agricultural Resource Center Beneficial Insects Research Unit Weslaco, TX 78596 Researchers: Sandra L. Brandt and Maureen K. Wright USDA, ARS, BCIRL Columbia, MO 65203 Elaine Backus USDA, ARS, PWA SJV Agricultural Sciences Cntr. Parlier, CA 93648 Wayne Hunter USDA, ARS, Horticultural Res. Lab Subtropical Insects Research Ft. Pierce, FL 34945

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• Biology And Ecology Of The Glassy-Winged Sharpshooter In The San Joaquin Valley
  Project Leaders: Kent M. Daane Division of Insect Biology University of California Berkeley, CA 94720

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• Effect Of Host Plant Fertilization On The Developmental Biology And Feeding Preference Of The Glassy-Winged Sharpshooter
  Project Leader: John A. Goolsby USDA, ARS Beneficial Insects Research Unit Weslaco, Texas 78596 Researcher: Mamoudou Sétamou USDA, ARS Beneficial Insects Research Unit Weslaco, Texas 78596 Cooperator: Joseph Patt USDA, ARS Beneficial Insects Research Unit Weslaco, Texas 78596

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• Dispersal And Movement Of The Glassy-Winged Sharpshooter And Associated Natural Enemies In A Continuous, Deficit-Irrigated Agricultural Landscape
  Project Leaders: Russell L. Groves USDA, ARS, SJVASC, CDPG Parlier, CA 93648 Marshall W. Johnson Department of Entomology University of California, Riverside Kearney Agricultural Center Parlier, CA 93648 Robert Luck Department of Entomology University of California Riverside, CA 92521 David Morgan CDFA, PDCP Riverside, CA 92501

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• Progress Toward Quantifying Landscape-Scale Movement Patterns Of The Glassy-Winged Sharpshooter And Its Natural Enemies Using A Novel Mark-Capture Technique
  Project Leaders: James Hagler, Jackie Blackmer, and Thomas Henneberry USDA, ARS Phoenix, AZ 85040 Cooperator: Vince Jones Washington State University Wenatchee, WA

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• Spatial Population Dynamics And Overwintering Biology Of The Glassy-Winged Sharpshooter In California’S San Joaquin Valley
  Project Leaders: Marshall W. Johnson Department of Entomology University of California, Riverside Kearney Agricultural Center Parlier, CA 93648 Elaine Backus USDA, ARS, PWA, SJVASC Parlier, CA 93648 Kent Daane Division of Insect Biology Department of ESPM University of California Berkeley, CA 94720 Youngsoo Son Department of Entomology University of California, Riverside Kearney Agricultural Center Parlier, CA 93648 Russell Groves USDA, ARS, PWA, SJVASC Parlier, CA 93648

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• Population Dynamics And Interactions Between Glassy-Winged Sharpshooter And Its Host Plants In Response To California Phenology
  Project Leaders: Russell F. Mizell, III, and Peter C. Andersen University of Florida, NFREC Quincy, FL 32351 Cooperators: Pascal Oevering UC Cooperative Extension Ventura, CA 93003 Marshall W. Johnson Kearney Agricultural Station Parlier, CA 93648 Ben Faber UC Cooperative Extension Ventura, CA 93003 Gary S. Bender UC Cooperative Extension San Diego, CA 92123

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• Relationship Between Olfactory And Visual Stimuli During Host Plant Recognition In Immature And Adult Glassy-Winged Sharpshooters
  Project Leaders: Joseph M. Patt and Mamoudou Sétamou USDA, ARS, Beneficial Insects Research Unit Weslaco, TX 78596 Research Personnel: Rosa I. Ruiz USDA, ARS, Beneficial Insects Research Unit Weslaco, TX 78596

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• Effects Of Feeding Substrate On Retention And Transmission Of Xylella Fastidiosa Strains By The Glassy-Winged Sharpshooter
  Project Leaders: Thomas M. Perring and Heather S. Costa Department of Entomology University of California Riverside, CA 92521 Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521

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• Evaluation Of Blue-Green Sharpshooter Flight Height
  Project Leader: Ed Weber UC Cooperative Extension Napa, CA 94559

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• Significance Of Riparian Plants As Reservoirs Of Xylella Fastidiosa For Infection Of Grapevines By The Blue-Green Sharpshooter
  Project Leader: Kendra Baumgartner USDA, ARS Davis, CA 95616 Cooperator: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720

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• Significance Of Riparian Plants As Reservoirs Of Xylella Fastidiosa For Infection Of Grapevines By The Blue-Green Sharpshooter
  Project Leader: Kendra Baumgartner USDA, ARS Davis, CA 95616 Cooperator: Alexander H. Purcell Division of Insect Biology University of California Berkeley, CA 94720

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• Reproductive Biology And Physiology Of Female Glassy-Winged Sharpshooters: Effect Of Host Plant Type On Fecundity And Development
  Project Leaders: Frank G. Zalom and Christine Y.S. Peng Department of Entomology University of California Davis, CA 95616 Cooperator: Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• Reproductive Biology And Physiology Of Female Glassy-Winged Sharpshooters: Morphology And Vitellogenesis Cycles
  Project Leaders: Frank G. Zalom and Christine Y.S. Peng Department of Entomology University of California Davis, CA 95616 Cooperator: Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• Xylem Chemistry Mediation Of Resistance To Pierce`S Disease
  Project Leaders: Peter C. Andersen University of Florida, NFREC Quincy, FL 32351 Cooperator: Maria L. Ishida University of Florida, NFREC Quincy, FL 32351

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• Quantitative Aspects Of The Transmission Of Xylella Fastidiosa By The Glassy-Winged Sharpshooter
  Project Leaders: Blake Bextine Department of Biology University of Texas Tyler, TX 75799 Matthew J. Blua and Thomas A. Miller Department of Entomology University of California Riverside, CA 92521

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• Aspects Of Pierce’S Disease Risk In Texas
  Project Leaders: Mark C. Black Department of Plant Pathology & Microbiology Texas A&M; University Agricultural Research and Extension Center Uvalde, TX 78802 Researchers: Alfred M. Sánchez and James L. Davis Department of Plant Pathology & Microbiology Texas A&M; University Agricultural Research and Extension Center Uvalde, TX 78802 James S. Kamas Department of Horticultural Sciences2 Texas A&M; University Fredericksburg, TX

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• Evaluation Of Single Nucleotide Polymorphisms And Sample Preparation Procedures In The Detection Of Xylella Fastidiosa Strains Important To California
  Project Leaders: Jianchi Chen and Edwin L. Civerolo USDA, ARS, PWA Crop Diseases, Pests, & Genetics Parlier, CA 93648

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• Culture-Independent Analysis Of Endophytic Microbial Communities In Grapevine In Relation To Pierce’S Disease
  Project Leaders: Donald A. Cooksey and James Borneman Department of Plant Pathology University of California Riverside, CA 92521

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• Role Of Type I Secretion In Pierce’S Disease
  Project Leader: Dean W. Gabriel Department of Plant Pathology University of Florida Gainesville, FL 32611 Researcher: Joseph D. Reddy Department of Plant Pathology University of Florida Gainesville, FL 32611

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• Evaluating The Roles Of Pili In Twitching And Long Distance Movement Of Xylella Fastidiosa In Grape Xylem And In The Colonization Of Sharpshooter Foregut
  Project Leaders: Harvey C. Hoch and Thomas J. Burr Department of Plant Pathology Cornell University, NYSAES Geneva, NY 14456 Cooperators: A.H. Purcell ESPM-Division of Insect Biology University of California Berkeley, CA 94720 Researcher: Yizhi Meng Department of Plant Pathology Cornell University, NYSAES Geneva, NY 14456 Steven E. Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720

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• Xylella Fastidiosa Outer Membrane
  Project Leader: Michele M. Igo Section of Microbiology University of California Davis, CA 95616 Cooperators: Ayumi Matsumoto Department of Food Science and Technology University of California Davis, CA 95616 Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616

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• Functional Genomics Of The Grape-Xylella Interaction: Towards The Identification Of Host Resistance Determinants
  Project Leader: Douglas Cook Department of Plant Pathology University of California Davis, CA 95616 Collaborators: Francisco Goes da Silva and Hong Kyu Choi Department of Plant Pathology University of California Davis, CA 95616 Alberto Iandolino University of California Davis, CA 95616 (Currently with Monsanto Corp.)

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• Functional Genomics Of The Grape-Xylella Interaction: Towards The Identification Of Host Resistance Determinants
  Project Leader: Douglas Cook Department of Plant Pathology University of California Davis, CA 95616 Collaborators: Francisco Goes da Silva and Hong Kyu Choi Department of Plant Pathology University of California Davis, CA 95616 Alberto Iandolino University of California Davis, CA 95616 (Currently with Monsanto Corp.)

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• Multi-Locus Simple Sequence Repeat (Ssr) Markers For Genotyping And Assessing Genetic Diversity Of Xylella Fastidiosa In California
  Project Leader: Hong Lin Crop Disease, Pest, & Genetics Research Unit USDA, ARS, SJVAC Parlier, CA 93648 Cooperators: Edwin Civerolo USDA, ARS, SJVAC Parlier, CA 93648 Russell Groves USDA, ARS, SJVAC Parlier, CA 93648 Andrew Walker Department of Viticulture & Enology University of California Davis, CA 95616

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• Effects Of Fimbrial (Fima, Fimf) And Afimbrial (Xada, Hxfb) Adhesins On The Adhesion Of Xylella Fastidiosa To Surfaces
  Project Leader: Steven E. Lindow Division of Plant and Molecular Biology University of California Berkeley, CA 94720 Researcher: Helene Feil University of California Berkeley, CA 94720

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• Role Of Unique Genes Of Xylella Fastidiosa Grape Strain In Host Specificity And Virulence To Grape And To Insect Using Microarray
  Project Leader: Steven E. Lindow Division of Plant and Molecular Biology University of California Berkeley, CA 94720 Cooperator: William S. Feil Division of Plant and Molecular Biology University of California Berkeley, CA 94720

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• Multi-Locus Sequence Typing (Mlst) To Identify Reservoirs Of Xylella Fastidiosa Diversity In Natural Hosts In California
  Project Leaders: Robert F. Luck, Richard Stouthamer, and Danel Vickerman Department of Entomology University of California Riverside, CA 92521

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• A Genome-Wide Approach To Plant-Host Pathogenicity In Xylella Fastidiosa: Multigenic Methods For Identifying Strains, For Studying The Role Of Inter-Strain Recombination, And For Identifying Pathogenicity Candidate Genes
  Project Leader: Leonard Nunney Department of Biology University of California Riverside, CA 92521 Cooperators: Richard Stouthamer and Robert Luck Department of Entomology University of California Riverside, CA 92521

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• Effects Of Chemical Milieu On Attachment, Aggregation, Biofilm Formation, And Vector Transmission Of Xylella Fastidiosa Strains
  Project Leader: Alexander H. Purcell ESPM-Division of Insect Biology University of California Berkeley CA 94720 Cooperators: Steven E. Lindow Department of Plant and Microbial Biology University of California Berkeley CA 94720 Researcher: Clytia Montllor Curley ESPM-Division of Insect Biology University of California Berkeley CA 94720 Harvey Hoch and Thomas Burr Department of Plant Pathology, NYSAES Barton Laboratory, Cornell University Geneva, NY 14456

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• A Screen For Xylella Fastidiosa Genes Involved In Transmission By Insect Vectors
  Project Leaders: Alexander H. Purcell ESPM-Division of Insect Biology University of California Berkeley, CA 94720 Researcher: Clelia Baccari ESPM-Division of Insect Biology University of California Berkeley, CA 94720

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• Patterns Of Xylella Fastidiosa Infection In Plants And Effects On Acquisition By Insect Vectors
  Project Leaders: Alexander H. Purcell ESPM – Division of Insect Biology University of California Berkeley, CA 94720 Researcher: Christina Wistrom ESPM – Division of Insect Biology University of California Berkeley, CA 94720

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• Documentation And Characterization Of Xylella Fastidiosa Strains In Landscape Hosts
  Project Leaders: Frank Wong and Donald A. Cooksey Department of Plant Pathology University of California Riverside, CA 92521 Heather S. Costa Department of Entomology University of California Riverside, CA 92521

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• Plasmid Addiction As A Novel Approach To Develop A Stable Plasmid Vector For Xylella Fastidiosa
  Project Leaders: Glenn M. Young Dept. of Food Science and Technology University of California Davis, CA 95616 Cooperators: Ayumi Matsumoto Dept. of Food Science and Technology University of California Davis, CA 95616 Michele Igo Section of Microbiology University of California Davis, CA 95616 Bruce Kirkpatrick Dept. of Plant Pathology University of California Davis, CA 95616

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• Funding Agencies
  Funding for this project was provided by the CDFA Pierce's Disease and Glassy-winged Sharpshooter Board. Table 1. Summary of physiological measurements for water relations and photosynthesis. Physiological parameterb Treatment plants ?p An,max gs Ci E

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• Responses Of Nicotiana Tabacum Cv. Sr-1 To Xylella Fastidiosa Strains
  Project Leaders: Marta Francis Department of Plant Pathology University of California Davis, CA 95616 Edwin L. Civerolo USDA, ARS SJV Ag. Sci. Center Parlier, CA 93648 George E. Bruening Department of Plant Pathology University of California Davis, CA 95616

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• Responses Of Nicotiana Tabacum Cv. Sr-1 To Xylella Fastidiosa Strains
  Project Leaders: Marta Francis Department of Plant Pathology University of California Davis, CA 95616 Edwin L. Civerolo USDA, ARS SJV Ag. Sci. Center Parlier, CA 93648 George E. Bruening Department of Plant Pathology University of California Davis, CA 95616

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• Comparative Study Of Xylella Fastidiosa Surface Proteins Exhibiting High Contents Cysteine Residues: Impact In Pathogenicity
  Project Leaders: Breno Leite CVSFR, Florida A&M; University Tallahassee, FL 32317 Peter C. Andersen and Maria L. Ishida NFREC, IFAS, University of Florida Quincy, FL 32351

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• Comparative Study Of Xylella Fastidiosa Surface Proteins Exhibiting High Contents Cysteine Residues: Impact In Pathogenicity
  Project Leaders: Breno Leite CVSFR, Florida A&M; University Tallahassee, FL 32317 Peter C. Andersen and Maria L. Ishida NFREC, IFAS, University of Florida Quincy, FL 32351

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• The Influence Of The Cell Suspension Redox Potential On The Capacity Of Xylella Fastidiosa To Aggregate
  Project Leaders: Breno Leite CVSFR, Florida A & M University Tallahassee, FL 32317 Peter C. Andersen NFREC, IFAS, University of Florida Quincy, FL 32351

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• The Influence Of The Cell Suspension Redox Potential On The Capacity Of Xylella Fastidiosa To Aggregate
  Project Leaders: Breno Leite CVSFR, Florida A & M University Tallahassee, FL 32317 Peter C. Andersen NFREC, IFAS, University of Florida Quincy, FL 32351

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• Identification Of Traits Of Xylella Fastidiosa Conferring Virulence To Grape And Insect Transmission By Analysis Of Global Gene Expression Using Dna Microarrays
  Project Leader: Steven Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Nian Wang and Bill Feil Department of Plant and Microbial Biology University of California Berkeley, CA 94720

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• Identification Of Traits Of Xylella Fastidiosa Conferring Virulence To Grape And Insect Transmission By Analysis Of Global Gene Expression Using Dna Microarrays
  Project Leader: Steven Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Nian Wang and Bill Feil Department of Plant and Microbial Biology University of California Berkeley, CA 94720

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• Genes Required For Type Iv Pili Formation And Twitching Motility In Xylella Fastidiosa
  Project Leaders: Y. Li, G. Hao, C. D. Galvani, Y. Meng, T. J. Burr, and H. C. Hoch Department of Plant Pathology Cornell University, NYSAES Geneva, NY 14456

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• Evaluation Of Signal Sequences For The Delivery Of Transgene Products Into The Xylem
  Project Leader: Abhaya M. Dandekar Department of Plant Sciences University of California Davis, CA 95616 Cooperators: David Gilchrist Department of Plant Pathology University of California Davis, CA 95616 Rejane Guimarães Department of Plant Pathology University of California Davis, CA 95616 John Labavitch Department of Plant Sciences University of California Davis, CA 95616 Cecilia Agüero Department of Plant Sciences University of California Davis, CA 95616

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• Evaluation Of Signal Sequences For The Delivery Of Transgene Products Into The Xylem
  Project Leader: Abhaya M. Dandekar Department of Plant Sciences University of California Davis, CA 95616 Cooperators: David Gilchrist Department of Plant Pathology University of California Davis, CA 95616 Rejane Guimarães Department of Plant Pathology University of California Davis, CA 95616 John Labavitch Department of Plant Sciences University of California Davis, CA 95616 Cecilia Agüero Department of Plant Sciences University of California Davis, CA 95616

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• Symbiotic Control Of Pierce’S Disease: Testing Reagents Against Xylella Fastidiosa
  Project Leader: Arinder K. Arora and Timothy S. Yolo Department of Entomology University of California Riverside, CA 92521 Collaborators: Carol Lauzon Department of Biological Sciences California State University, Hayward, CA 94542 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 David Lampe Department of Biological Sciences Duquesne University Pittsburg, PA 19219 Consultant: Frank Richards Yale Medical School New Haven, CT 06520

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• Exploiting Xylella Fastidiosa Proteins For Pierce’S Disease Control
  Project Leaders: George Bruening Department of Plant Pathology University of California Davis, CA 95616 Cooperators: Paul Feldstein and Marta Francis Department of Plant Pathology University of California Davis, CA 95616 Edwin L. Civerolo USDA, ARS San Joaquin Valley Agricultural Sciences Center Parlier, CA 93648 Abhaya M. Dandekar Department of Pomology University of California Davis, CA 95616 Goutam Gupta MS M888, Biol. Division Los Alamos National Lab. Los Alamos, NM 87544

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• Siderophore Production Of Alcaligenes Xylosoxidans Denitrificans And Potential Biological Control Against Xylella Fastidiosa
  Project Leaders: Surachet Chareonkajonchai CMDB Graduate Program University of California Riverside, CA 92521 Collaborators: Carol Lauzon Department of Biological Sciences California State University Hayward, CA 94542 Paulo Teixeira Lacava Department of Entomology University of California Riverside, CA 92521 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 Consultant: Frank Richards Yale University New Haven, CT 06520

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• Effects Of Group, Cultivar, And Climate On The Establishment And Persistence Of Xylella Fastidiosa Infections Causing Almond Leaf Scorch
  Project Leaders: Kent M. Daane and Alexander Purcell Department of Environmental Science, Policy and Management University of California Berkeley, CA 94720 Cooperating Researcher: Christina Wistrom Department of Environmental Science, Policy and Management University of California Berkeley CA 94720

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• Importance Of Ground Vegetation In The Dispersal And Overwintering Of Xylella Fastidiosa
  Project Leaders: Kent M. Daane and Alexander Purcell Division of Insect Biology Dept. of Environ. Science, Policy, & Management University of California Berkeley, CA 94720 Cooperators: Elaine Shapland, Christina Wistrom, and Glenn Yokota Division of Insect Biology University of California Berkeley, CA 94720 Joe Connell, Roger Duncan, and Mario Viveros UC Cooperative Extension Butte Co., Stanislaus Co., and Kern Co., respectively

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• Design Of Chimeric Anti-Microbial Proteins For Rapid Clearance Of Xylella
  Project Leaders: Abhaya M. Dandekar Department of Pomology University of California Davis, CA 95616 Elizabeth Hong-Geller B-1, MS M888, LANL Los Alamos, NM 87545 Collaborators: George Bruening Department of Plant Pathology University of California Davis, CA 95616 Edwin L. Civerolo USDA, ARS Parlier, CA 93468 Goutam Gupta B-1, MS M888, LANL Los Alamos, NM 87545 Karen McDonald Chem. Engr. and Material Sci. University of California Davis, CA 95616

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• Evaluation Of Grapevine Endophytic Bacteria For Control Of Pierce’S Disease
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Cooperators: Cheryl Whistler and Margot Wilhelm Department of Plant Pathology University of California Davis, CA 95616

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• Identification Of Mechanisms Mediating Cold Therapy Of Xylella Fastidiosa- Infected Grapevines
  Project Leader: Bruce Kirkpatrick Department of Plant Pathology University of California Davis, CA 95616 Cooperator: Melody Meyer Department of Plant Pathology University of California Davis, CA 95616

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• Interaction Between Xylella Fastidiosa And Curtobacterium Flaccumfaciens, An Endophytic Bacterium
  Project Leaders: Paulo T. Lacava Department of Entomology University of California Riverside, CA 92521 Collaborators: Wen B. Li Fruit Laboratory USDA Beltsville, MD 20705 João Lúcio Azevedo Department of Genetics University of São Paulo Piracicaba, SP 13400-970 Welington L. Araújo Department of Genetics University of São Paulo Piracicaba, SP 13400-970 John S. Hartung Fruit Laboratory USDA Beltsville, MD 20705 Thomas A. Miller Department of Entomology University of California Riverside, CA 92521

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• Isolation And Functional Testing Of Pierce’S Diseasespecific Promoters From Grape
  Project Leaders: David Gilchrist, Douglas Cook, and James E. Lincoln Department of Plant Pathology University of California Davis, CA 95616

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• Soluble Forms Of An Anti-Xylella Antibody And Strains Of Alcaligenes Xylosoxidans Denitrificans Capable Of Secreting Them
  Project Leader: David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219 Consultants: Frank Richards Yale University New Haven, CT 06520 Collaborators: Carol Lauzon Department of Biological Sciences California State University Hayward, CA 94542 Bob Rose Frederick, MD Thomas A. Miller Department of Entomology University of California Riverside, CA 92521

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• Ecological And Genetic Characteristics Associated With Alcaligenes Xylosoxidans Denitrificans
  Project Director: Carol R. Lauzon Department of Biological Sciences California State University East Bay Hayward, CA 94568 Collaborators: Thomas A. Miller Department of Entomology University of California Riverside, CA 92551 Research Personnel: Ranjana Ambannavar and Lavanya Telunktula California State University Hayward, CA 94568

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• Management Of Pierce’S Disease Of Grape By Interfering With Cell-Cell Communication In Xylella Fastidiosa
  Project Leader: Steven E. Lindow Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Cooperators: Subhadeep Chatterjee and Karyn Newman Department of Plant and Microbial Biology University of California Berkeley, CA 94720 Alexander Purcell Department of Environmental Science, Policy, & Management University of California Berkeley, CA 94720

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• Environmental Fate Of A Genetically Marked Endophyte In Grapevines
  Project Leader: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 Consultants: Frank Richards Yale University New Haven, CT 06520 Collaborators: Carol Lauzon Department of Biological Sciences California State University Hayward, CA 94542 Bob Rose Frederick, MD David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219

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• Genotypic Characterization Of Alcaligenes Xylosoxidans Subsp. Denitrificans (Axd Hc01) And Four Related Strains
  Project Leader: Jennifer Parker Department of Entomology University of California Riverside, CA 92521 Cooperators: Carol R. Lauzon Department of Biological Sciences California State University Hayward, CA 95452 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 David J. Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 15282

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• Development Of A Field Sampling Plan For Glassy-Winged Sharpshooter Vectored Pierce’S Disease
  Project Leaders: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Cooperators: Yong-Lak Park Department of Entomology University of California Riverside, CA 92521 Murry P. Pryor Cooperative Extension University of California Bakersfield, CA 93307 Jennifer Hashim UC Cooperative Extension Bakersfield, CA 93307 Carmen Gispert UC Cooperative Extension Indio, CA 92201

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• Fate Of A Genetically Modified Bacterium In The Foregut Of The Glassy-Winged Sharpshooter
  Project Leader: Jose Luis Ramirez Department of Entomology University of California Riverside, CA 92521 Collaborators: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 Carol Lauzon Department of Biological Sciences California State University Hayward, CA 94542 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219

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• Fate Of Xylella Fastidiosa In The Foregut Of Glassy-Winged Sharpshooters Fed On Two Host Plants
  Project Leader: Jose Luis Ramirez Department of Entomology University of California Riverside, CA 92521 Collaborators: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Project Director: Thomas A. Miller Department of Entomology University of California Riverside, CA 92521 Carol Lauzon Department of Biological Sciences California State University Hayward, CA 94542 David Lampe Department of Biological Sciences Duquesne University Pittsburgh, PA 19219

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• Evaluation Of Genes Isolated By A Functional Genetic Screen For Suppression Of Bacterial Growth Or Symptoms In Pierce’S Disease
  Project Leaders: David Gilchrist and James E. Lincoln Department of Plant Pathology University of California Davis, CA 95616

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• Inhibition Of Xylella Fastidiosa Biofilm Formation Via Metal Chelators
  Project Leaders: Mipha L. Koh and Jeffrey H. Toney Department of Chemistry and Biochemistry Montclair State University Montclair, New Jersey

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• Inhibition Of Xylella Fastidiosa Biofilm Formation Via Metal Chelators
  Project Leaders: Mipha L. Koh and Jeffrey H. Toney Department of Chemistry and Biochemistry Montclair State University Montclair, New Jersey

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• Manipulation Of Hirsutella As A Biological Control Of The Glassy-Winged Sharpshooter
  Project Leaders: Drion Boucias University of Florida Gainesville, FL 32611 Cooperators: Verena Blaeske University of Florida Gainesville, FL 32611

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• Characterization Of Neonicotinoids And Their Plant Metabolites In Citrus Trees And Grapevines, And Evaluation Of Their Efficacy Against The Glassy-Winged Sharpshooter And The Egg Parasitoid Gonatocerus Ashmeadi
  Project Leaders: Frank J. Byrne and Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• Evaluation Of Resistance Potential In The Glassy-Winged Sharpshooter Using Toxicological, Biochemical, And Genomics Approaches
  Project Leaders: Frank Byrne, Nick Toscano, Brian Federici, and Cynthia LeVesque Dept of Entomology, University of California, Riverside, CA 92521

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• Development Of Molecular Diagnostic Markers For Glassy-Winged And Smoketree Sharpshooters For Use In Predator Gut Content Examinations
  Project Leaders: Jesse H. de León USDA, ARS BIRU Weslaco, Texas 78596 James Hagler, Valerie Fournier USDA, ARS Western Cotton Research Lab Phoenix, AZ 85040 Kent Daane Division of Insect Biology University of California Berkeley, CA 94720

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• The Utility Of Inter-Simple Sequence Repeat-Polymerase Chain Reaction (Issr-Pcr) To Distinguish Geographic Populations Of The Smoke-Tree Sharpshooter And Egg Parasitoid Species Of The Genus Gonatocerus
  Project Leader: Jesse H. de León USDA, ARS Beneficial Insect Research Unit Weslaco, TX 78596 Cooperators: James R. Hagler USDA, ARS Western Cotton Res. Lab. Phoenix, AZ 85040 Guillermo Logarzo USDA, ARS South American Biological Control Lab. Buenos Aires, Argentina David J. W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501

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• Discovery Of A Cryptic Species Complex In Gonatocerus Morrilli, A Primary Egg Parasitoid Of The Glassy-Winged Sharpshooter
  Project Leaders: Jesse H. de León, Walker A. Jones, and Mamoudou Sétamou USDA, ARS Beneficial Insects Research Unit Weslaco, TX 78596 David J.W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501

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• Small Scale Post-Release Evaluation Of A Gonatocerus Morrilli Program In California Against The Glassy-Winged Sharpshooter: Utility Of Developed Molecular Diagnostic Tools
  Project Leaders: Jesse H. de León USDA, ARS Beneficial Insects Research Unit Weslaco, TX 78596 David J.W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501

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• The Alimentary Track Of The Glassy-Winged Sharpshooter As A Target For Control Of Pierce’S Disease And Development Of Mimetic Insecticidal Peptides For Glassy-Winged Sharpshooter Control
  Project Leader: Brian A. Federici Department of Entomology University of California Riverside, CA 92521

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• Identifying Key Predators Of The Various Glassy-Winged Sharpshooter Lifestages
  Project Leaders: Valerie Fournier Division of Insect Biology University of California Weslaco, TX 78596 Jesse de León Beneficial Insects Research Unit USDA, ARS Weslaco, TX 78596 Cooperators: Nilima Prabhaker and Heather Costa Department of Entomology University of California Riverside, CA 92521

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• Exploration For Biological Control Agents In The Native Range Of The Glassy-Winged Sharpshooter
  Project Leader: John Goolsby USDA, ARS, Beneficial Insects Research Unit Weslaco, TX 78596 Researcher: Mamoudou Sétamou USDA, ARS, Beneficial Insects Research Unit Weslaco, TX 78596

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• Epidemiology Of Pierce’S Disease In The Central San Joaquin Valley Of California: Factors Affecting Pathogen Distribution And Movement
  Project Leaders: Russell L. Groves and Jianchi Chen USDA, ARS, SJVASC, CDPG Parlier, CA 93648 Collaborators: Ed Civerolo USDA, ARS, SJVASC Parlier, CA 93648 Daniel Bigham Tulare County Agricultural Commissioner Office Tulare, CA 93274 Kent Daane Division of Insect Biology University of California Berkeley, CA 94720 Hong Lin USDA, ARS, SJVASC, CDPG Parlier, CA 93648 Marshall Johnson Department of Entomology University of California Riverside, CA 92521

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• Progress Toward Developing A Novel Immunological Approach For Quantifying Predation Rates On The Glassy-Winged Sharpshooter
  Project Leaders: James Hagler and Thomas Henneberry USDA, ARS Phoenix, AZ 85040 Valerie Fournier and Kent Daane University of California Berkeley, CA 94720 Cooperator: David Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501

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• Oviposition And Native Parasitoids Of The Blue-Green Sharpshooter, And Host Specificity Of Gonatocerus Ashmeadi On The Smoketree Sharpshooter And The Blue-Green Sharpshooter
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 Researcher: Elizabeth A. Boyd Department of Entomology University of California Riverside, CA 92521

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• Realized Lifetime Parasitism Of Glassy-Winged Sharpshooter Egg Masses By Gonatocerus Ashmeadi
  Project Leader: Mark S. Hoddle Department of Entomology University of California Riverside, CA 92521 Cooperators: Nic Irvin and Robert Luck Department of Entomology University of California Riverside, CA 92521

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• Reproductive And Developmental Biology Of Gonatocerus Ashmeadi, An Egg Parasitoid Of The Glassy-Winged Sharpshooter
  Project Leader: Mark Hoddle Department of Entomology University of California Riverside, CA 92521 Cooperator: Leigh Pilkington Department of Entomology University of California Riverside, CA 92521

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• Is The Glassy-Winged Sharpshooter Parasitoid Gonatocerus Morrilli One Species Or A Complex Of Closely Related Sibling Species?
  Project Leaders: Mark S. Hoddle and Richard Stouthamer Dept. of Entomology University of California Riverside, CA 92521 Cooperators: Serguei Triapitsyn and Herman van Oosten Dept. of Entomology University of California Riverside, CA 92521

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• Maintaining And Evaluating Quarantine Cultures Of Gonatocerus Spp., Promising Egg Parasitoids From Argentina And Mexico, For The Classical Biological Control Of The Glassy-Winged Sharpshooter In California
  Project Leaders: Mark S. Hoddle and Serguei Triapitsyn Department of Entomology University of California Riverside, CA 92521 Cooperators: David J. W. Morgan CDFA, PDCP Mt. Rubidoux Field Station Riverside, CA Guillermo A. Logarzo USDA, ARS South American Biological Control Laboratory Washington, D.C.

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• Environmental Risk Assessment Of Egg Parasitoids From South America: Nontarget Field And Laboratory Host Range In Argentina And The United States
  Project Leaders: Walker A. Jones (Coordinator) USDA, ARS European Biological Control Laboratory Montferrier-sur-Lez, France Eduardo G. Virl CONICET-PROIMI Avda Belgrano y Pasaje Caseros S. M. de Tucumán, Argentina Guillermo A. Logarzo USDA, ARS South American Biological Control Laboratory Buenos Aires, Argentina Erica Luft CONICET-PROIMI Avda Belgrano y Pasaje Caseros S. M. de Tucumán, Argentina

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• Evaluation Of Some Fungal Pathogens For The Control Of The Glassy-Winged Sharpshooter
  Project Leader: Harry K. Kaya Department of Nematology University of California Davis, CA 95616 Cooperator: Michael R. McGuire USDA, ARS Shafter Research and Extension Center Shafter, CA 93263

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• The Influence Of Temperature On Development And Reproduction Of The Egg Parasitoid Gonatocerus Ashmeadi
  Project Leader: Roger A. Leopold USDA, ARS Biosciences Research Laboratory Fargo, ND 58105 Cooperators: Wenlong Chen Department of Entomology Fargo, ND 58105 David J. Morgan CDFA, PDCP Mt. Rubidoux Field Station Riverside, CA

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• Refrigerated Storage Of Glassy-Winged Sharpshooter Eggs Used For Propagation Of The Parasitoid, Gonatocerus Ashmeadi
  Project Leader: Roger A. Leopold USDA, ARS Biosciences Research Laboratory Fargo, ND 58105 Cooperator: Wenlong Chen Department of Entomology North Dakota State University Fargo, ND 58105

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• The Effect Of Dormant Season Survival Of Xylella Fastidiosa In Grapevines On Pierce’S Disease Epidemics In California
  Project Leader: Barry L. Hill CDFA, PDCP Sacramento, CA 95832 Cooperators: Jennifer Hashim UC Cooperative Extension Kern County, CA William Peacock UC Cooperative Extension Tulare County, CA

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• Glassy-Winged Sharpshooter’S (Gwss) Population Dynamics As A Means Of Gaining Insight Into The Management Of Gwss Populations
  Project Leader: Robert F. Luck Department of Entomology University of California Riverside, CA 92521 Cooperators: Carlos E. Coviella Laboratorio de Ecología Universidad Nacional de Luján, (6700) Luján, Argentina Peter Andersen and Russell Mizell, III Southern Region Pest Management Center University of Florida Quincy, FL 32351

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• Effects Of Juvenile Hormone Analogs On Survival And Reproduction Status Of The Glassy-Winged Sharpshooter
  Project Leaders: Russell F. Mizell, III, and Peter C. Andersen University of Florida, NFREC Quincy, FL Cooperators: Chris Tipping and Tobin Northfield University of Florida, NFREC Quincy, FL 32351 Wayne Hunter USDA, ARS Ft. Pierce, FL, 34945

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• Mycopathogens And Their Exotoxins Infecting The Glassy-Winged Sharpshooter: Survey, Evaluation, And Storage.
  Project Leaders: Russell F. Mizell, III University of Florida, NFREC Quincy, FL 32351 Cooperator: Chris Tipping University of Florida, NFREC Quincy, FL 32351

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• Seasonal Population Dynamics Of Glassy-Winged Sharpshooter Egg Parasitoids: Variability Across Sites And Host Plants
  Project Leader: Joseph G. Morse Department of Entomology University of California Riverside, CA 92521 Cooperators: David J. W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501 Rodney Mendes CDFA, PDCP Riverside, CA 92507 Jonathan M. Lytle Department of Entomology University of California Riverside, CA 92521 Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• The Anagrus Epos Complex: A Likely Source Of Effective Classical Biological Control Agents For Glassy-Winged Sharpshooter Control
  Project Leaders: Joseph G. Morse and Richard Stouthamer Department of Entomology University of California Riverside, CA 92521 Cooperators: Serguei Triapitsyn Department of Entomology University of California Riverside, CA 92521 Rodney Mendes CDFA, PDCP Riverside, CA 92507 David J.W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501 Jonathan M. Lytle and Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• The Area-Wide Pest Management Of Glassy-Winged Sharpshooter In Tulare County
  Project Team: Beth Stone-Smith USDA, APHIS, PPQ, WR Arvin, CA 93203 Bill Appleby Tulare County Tulare, CA 93274 Judy Stewart-Leslie GWSS Area-Wide Program Exeter, CA 93221 Don Borges Tulare County Tulare, CA 93274 Gary Kunkel Tulare County Tulare, CA 93274 Dennis Haines Tulare County Tulare, CA 93274 Sean Hardy CDFA, PDCP Fresno, CA Jesse Churchill CDFA, PDCP Tulare, CA 93274

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• Laboratory And Field Evaluations Of Neonicotinoid Insecticides Against The Glassy-Winged Sharpshooter
  Project Leaders: Nick Toscano and Frank Byrne Department of Entomology University of California Riverside, CA 92521 Cooperators: Mac Learned, Ed Ishida, and Manuel Jiminez Bayer CropScience Carmen Gispert UC Cooperative Extension Riverside County Riverside, CA Ben Drake Drake Enterprises Temecula, CA

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• Riverside County Glassy-Winged Sharpshooter Area-Wide Management Program In The Coachella And Temecula Valleys
  Project Leaders: Nick C. Toscano Department of Entomology University of California Riverside, CA 92521 Cooperators: Rodney Mendes CDFA, PDCP Riverside, CA 92507 Robert Mulherin Riverside County Riverside, CA 92502

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• Compatibility Of Select Insecticides With Natural Enemies Of The Glassy-Winged Sharpshooter And Other Pests
  Project Leaders: Nick C. Toscano and Joseph G. Morse Department of Entomology University of California Riverside, CA 92521 Cooperators: Nilima Prabhaker Department of Entomology University of California Riverside, CA 92521 Timothy J. Henneberry WCRL, USDA, ARS Phoenix, AZ 85040

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• Preparing And Submitting For Publication A Pictorial, Annotated Key To Gonatocerus Species And Other Genera And Species Of Mymaridae (Hymenoptera) – Egg Parasitoids Of Homalodisca Spp. And Other Proconiine Sharpshooters In North America, With Emphasis On The Species Native Or Introduced To California
  Project Leader: Serguei V. Triapitsyn Department of Entomology University of California Riverside, CA 92521 Cooperators: David J.W. Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA Danel B. Vickerman Department of Entomology University of California Riverside, 92521

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• Optimization Of Admire Applications In North Coast Vineyards
  Project Leaders: Ed Weber UC Cooperative Extension Napa, CA 94559 Frank J. Byrne and Nick C. Toscano Department of Entomology University of California Riverside, CA 92521

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• Glassy-Winged Sharpshooter Impact On ‘Washington’ Navel Orange Yield, Fruit Size, And Quality
  Project Leader: Raymond L. Hix Center for Viticulture and Small Fruit Research Florida Agricultural and Mechanical University Tallahassee, FL Cooperators: Mary Lu Arpaia Department of Botany and Plant Science University of California, Riverside Kearny Agricultural Center Parlier, CA Beth Grafton-Cardwell Department of Entomology University of California, Riverside Kearny Agricultural Center Parlier, CA

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• Linking The Model Of The Development Of Pierce’S Disease In Grapevines To An Understanding Of The Dynamics Of Glassy-Winged Sharpshooter Transmission Of Xylella Fastidiosa To Grapevines And Grapevine Gene Expression Markers Of Pierce’S Disease
  Project Leaders: John Labavitch Dept. of Plant Sciences University of California Davis, CA 95616 Ken A. Shackel Dept. of Plant Sciences University of California Davis, CA 95616 Cooperators: L. Carl Greve Dept. of Plant Sciences University of California Davis, CA 95616 Alonso Pérez Dept. of Plant Sciences University of California Davis, CA 95616 Bruce Kirkpatrick Dept. of Plant Pathology University of California Davis, CA 95616 Caroline Roper Dept. of Plant Pathology University of California Davis, CA 95616 David Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501 Elaine Backus Exotic Pests and Diseases Lab USDA, ARS Parlier, CA 93648 Mark Matthews Dept. of Viticulture and Enology University of California Davis, CA 95616

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• Characterization And Identification Of Pierce’S Disease Resistance Mechanisms: Analysis Of Xylem Anatomical Structures And Of Natural Products In Xylem Sap Among Vitis
  Project Leader: Hong Lin Crop Diseases, Pests, and Genetics Research Unit USDA, ARS Parlier, CA 93648 Cooperators: Felix Fritschi Crop Diseases, Pests, and Genetics Research Unit USDA, ARS Parlier, CA 93648 Andrew Walker Department of Viticulture and Enology University of California Davis, CA, 95616

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• Developing Transcriptional Profiles And Gene Expression Analysis Of Grape Plant Response To Xylella Fastidiosa
  Project Leader: Hong Lin Crop Diseases, Pests, and Genetics USDA, ARS Parlier, CA 93648 Cooperators: Harsha Doddapaneni and Andrew Walker Department of Viticulture and Enology University of California Davis, CA, 95616

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• Comparative Proteomic Analysis Of Stem Tissue And Xylem Sap From Pierce’S Disease Resistant And Susceptible Grapevines
  Project Leaders: Hong Lin Crop Diseases, Pests, and Genetics Research Unit USDA, ARS Parlier, CA 93648 Cooperators: Litao Yang Crop Diseases, Pests and Genetics Research Unit USDA, ARS Parlier, CA 93648 Felix Fritschi Crop Diseases, Pests, and Genetics Research Unit USDA, ARS Parlier, CA 93648 Andrew Walker Department of Viticulture and Enology University of California Davis, CA, 95616

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• Epidemiological Analyses Of Glassy-Winged Sharpshooter And Pierce'S Disease Data
  Project Leader: Thomas M. Perring Department of Entomology University of California Riverside, CA 92521 Cooperators: Yong-Lak Park, Charles A. Farrar, and Rayda K. Krell Department of Entomology University of California Riverside, CA 92521 Barry Hill CDFA, PDCP Sacramento, CA 95814 Maggi Kelly Deparment of Environmental Sciences, Policy and Management University of California Berkeley, CA 94720

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• Improving Our Understanding Of Substance Transport Across Graft Unions
  Project Leaders: Bruce Reisch Department of Horticultural Sciences NYSAES- Cornell University Geneva, NY 14456 Cooperators: Seok-Hyun Eom and Julie R. Kikkert Cornell University NYS Agric. Experiment Station Geneva, NY 14456

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• Testing Transgenic Grapevines For Resistance To Pierce’S Disease
  Project Leaders: Bruce I. Reisch and Julie Kikkert Department of Horticultural Sciences NYSAES, Cornell University Geneva, NY 14456 Cooperators: Andrew Walker Department of Viticulture and Enology University of California Davis, CA 95616 Claire-Lise Rosenfield Department of Horticultural Sciences NYSAES, Cornell University Geneva, NY 14456

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• Mechanisms Of Pierce’S Disease Transmission In Grapevines: The Xylem Pathways And Movement Of Xylella Fastidiosa. Xylem Structure And Connectivity In Grapevine Shoots Suggests A Passive Mechanism For The Systemic Spread Of Bacteria In Grape Plants Infected With Pierce’S Disease
  Project Leaders: Thomas L. Rost Section of Plant Biology University of California Davis, CA 95616 Cooperator: David S. Chatelet Section of Plant Biology University of California Davis, CA 95616

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• Magnetic Resonance Imaging: A Non-Destructive Approach For Detection Of Xylem Blockages In Xylella Fastidiosa Infected Grapevines
  Project Leaders: Ken Shackel and John Labavitch Department of Plant Sciences University of California Davis, CA 95616 Cooperators: Mark Matthews Dept. of Vitic. and Enology University of California Davis, CA 95616 L. Carl Greve Dept. of Plant Sciences University of California Davis, CA 95616 Jeffrey Walton NMR Facility University of California Davis, CA 95616 Alonso Pérez Dept. of Plant Sciences University of California Davis, CA 95616

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• Map-Based Identification And Positional Cloning Of Xylella Fastidiosa Resistance Genes From Known Sources Of Pierce’S Disease Resistance In Grape
  Project Leaders: Andrew Walker and Summaira Riaz Department of Viticulture and Enology University of California Davis, CA 95616

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• Breeding Pierce’S Disease Resistant Winegrapes
  Project Leaders: Andrew Walker and Alan Tenscher Dept. of Viticulture and Enology University of California Davis, CA 95616-8749

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• Variation Of Xylella Fastidiosa Colonization In Tolerant And Susceptible Grape Cultivars
  Dulce Carbajal and Lisa Morano Department of Natural Sciences University of Houston Downtown Houston, TX 77002

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• Where, When And How Do Ingestion And Other Feeding Behaviors Of The Glassy-Winged Sharpshooter Allow Inoculation Of Xylella Fastidiosa?
  Project Leader: Elaine Backus USDA, ARS, PWA Crop Diseases, Pests & Genetics Parlier, CA 93648 Cooperators: William H. Bennett Private Consultant Otterville, MO 65348 David Morgan CDFA, PDCP Mount Rubidoux Field Station Riverside, CA 92501 Personnel: P. Houston Joost Dept. of Entomology University of California Riverside, CA 92521 Thomas Miller Department of Entomology University of California Riverside, CA 92521 Jianchi Chen USDA, ARS, PWA Crop Diseases, Pests & Genetics Parlier, CA 93648 Holly Shugart USDA, ARS, PWA Crop Diseases, Pests & Genetics Parlier, CA 93648 Gregory Walker Department of Entomology University of California Riverside, CA 92521

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